diff --git a/Lib/test/test_subprocess.py b/Lib/test/test_subprocess.py
index 7d0708d5f7fe64..a0d3dcd069a97f 100644
--- a/Lib/test/test_subprocess.py
+++ b/Lib/test/test_subprocess.py
@@ -651,6 +651,7 @@ def is_env_var_to_ignore(n):
             # on adding even when the environment in exec is empty.
             # Gentoo sandboxes also force LD_PRELOAD and SANDBOX_* to exist.
             return ('VERSIONER' in n or '__CF' in n or  # MacOS
+                    '__PYVENV_LAUNCHER__' in n or # MacOS framework build
                     n == 'LD_PRELOAD' or n.startswith('SANDBOX') or # Gentoo
                     n == 'LC_CTYPE') # Locale coercion triggered