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Documentation: Missing test files and errors when running commands from tutorial #693

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bentyeh opened this issue Feb 27, 2025 · 1 comment

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@bentyeh
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bentyeh commented Feb 27, 2025

The "Advanced Step-by-step peak calling using MACS3 commands" tutorial [webpage, source] has 2 issues:

  1. The tutorial claims that the 2 test files, CTCF_ChIP_200K.bed.gz and CTCF_Control_200K.bed.gz, can be found "in the MACS3 GitHub repository in the test directory." However, there are no such files currently in the master branch of the MACS GitHub repository.

  2. The macs3 pileup -i <*.bed> ... commands currently (using macs3 3.0.3) raise an error

    ERROR @ 26 Feb 2025 21:53:52: [34 MB] Format "AUTO" cannot be recognized!
    

    and seem to fail to automatically detect the BED input format. Adding the option -f BED resolves the issue.

    As I'm new to MACS, I'm not sure if this is simply a documentation issue (i.e., the commands in the tutorial should be updated to include the -f BED option) or if this reflects a bug in the macs3 pileup program.

@taoliu
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taoliu commented Feb 27, 2025

@bentyeh Thanks for the feedback! I need to revise that document. Stay tuned.

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